FIGURE 6.

Single neuron response. (A) Fluorescent analysis of video taken from a 10× field of view. The image is the average intensity of the fluorescent signal across four events of 450 frames each. Green contour encloses areas that were spontaneously active during the first event (at 4.5 μM of CNQX, the highest concentration at which spontaneous bursting activity persisted across the whole culture). Red points represent locations that responded to TvMS during the last three events (at CNQX concentration of 9 μM, for which no bursting activity was observed). (B) Traces of the fluorescence signal (in units of the relative change of intensity as a fraction of the background intensity) during the four events: green traces (top row), average intensity of all coordinates enclosed in the green contour; and red traces (bottom row), average intensity of all red coordinates. TvMS pulses are marked with a dashed vertical line, with the intensity of the induced electric field indicated in V/m.