FIGURE 1.

The ¹H-¹⁵N HSQC spectrum of uniformly ¹⁵N-labeled rmMBP (176 residues, including a C-terminal LEH₆ tag) dissolved in 100 mM KCl in water at a concentration of 1.75 mM, recorded at 300 K. A total of 160 of 164 expected backbone peaks are assigned (there are 11 prolyl residues); peaks that appear below the displayed contour levels are not shown. Assignments are based on the assignments first performed at 277 K (56), and were obtained by tracking the assigned crosspeaks through a series of spectra recorded at increasingly higher temperatures.