FIGURE 10.

Comparison of the secondary structure propensity (SSP) of (A) rmMBP alone at 277 K, and (B) rmMBP bound with CaM at 300 K. Both samples were dissolved in 100 mM KCl, pH 6.5, 10% D₂O, and 8 mM CaCl₂ was included with the CaM-containing sample. For both conditions, the SSP was calculated on the basis of the C^α and C^β shifts (in contrast to Fig. 3). The shifts were re-referenced as described by Marsh et al. (68) before the calculation of the SSP scores. The SSP score is interpreted as the percentage of conformers in the disordered ensemble that exhibit ordered secondary structure at a given residue (68).