FIGURE 2.

Chemical shift difference plots (A) H^N, (B) N, (C) H^α, (D) C^β, (E) C^α, for rmMBP in 30% TFE-d₂ at 300 K. The random coil values for H^N, N, H^α, and C^α have been adjusted for sequence dependence as previously described for the Golli-MBP isoform rmBG21 (64). The dashed lines in all the panels indicate the minimum threshold values for the formation of secondary structure elements. Generally, a consecutive stretch of four residues above the threshold value, or three residues below the threshold value, is considered to be an α-helical or β-strand element, respectively.