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. 2008 Mar 7;94(12):4847–4866. doi: 10.1529/biophysj.107.125823

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Copyright © 2008, Biophysical Society

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Chemical shift perturbation (CSP) plot of the weighted average of the H^N and N chemical shift deviations from random coil values. (A) rmMBP alone at 277 K; (B) rmMBP alone at 300 K; (C) rmMBP bound with CaM at 300 K; (D) difference between the CSP of rmMBP at 277 K and rmMBP recorded at 300 K; and (E) difference between the CSP of rmMBP at 277 K and rmMBP bound to CaM recorded at 300 K. The solute for all experiments was 100 mM KCl, pH 6.5, 10% D₂O, with 8 mM CaCl₂ added to the CaM-containing samples. The weighted average of a specific residue was calculated as (129).

Inline graphic — Chemical shift perturbation (CSP) plot of the weighted average of the H^N and N chemical shift deviations from random coil values. (A) rmMBP alone at 277 K; (B) rmMBP alone at 300 K; (C) rmMBP bound with CaM at 300 K; (D) difference between the CSP of rmMBP at 277 K and rmMBP recorded at 300 K; and (E) difference between the CSP of rmMBP at 277 K and rmMBP bound to CaM recorded at 300 K. The solute for all experiments was 100 mM KCl, pH 6.5, 10% D₂O, with 8 mM CaCl₂ added to the CaM-containing samples. The weighted average of a specific residue was calculated as (129).