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. 2008 Mar 7;94(12):4948–4956. doi: 10.1529/biophysj.107.124107

FIGURE 4.

FIGURE 4

Results of using the a/LCI analysis technique to measure size of spheroidal particles by using angular scattering distributions calculated using the T-matrix model as input into the analysis. Angular scattering distributions correspond to a particle with an equal volume radius of 3.75 μm, a background refractive index of 1.36, a target refractive index of 1.42, and a size distribution of 2.5%. The polarization and orientation of the light and particle respectively are (a) S11, α = 90°, β = 90°; (b) S22, α = 90°, β = 90°, (c) S11, α = 0°, β = 60°, and (d) S22, α = 0°, β = 90°, where α and β are Euler angles and S11 and S22 are parallel and perpendicular incident polarizations, respectively. The equatorial and polar manifolds are 1 wavelength (830 nm) thick, with the center corresponding to the equatorial and polar axes of the spheroid at that aspect ratio. These results indicate that the size measurement of a spheroidal particle as determined by the a/LCI analysis technique almost universally falls on either the equatorial or polar manifold; however, there is no method of consistently predicting which it will be.