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Structural comparison between mesophilic EcDHFR (solid representation) and thermophilic BsDHFR (shaded representation), (a) in cartoon and (b) in space-filling van der Waals representation. (c) The Cα-atom crystallographic B factors are plotted for both enzymes. The Ec and Bs structural coordinates and B factors were obtained from the Protein Data Bank (43) accession codes 2ANQ (16) and 1ZDR (15), respectively. Both structures were determined at the same temperature, 100 K.
