FIGURE 6.

The microtubule cytoskeleton in HeLa cells. Axial maximum-intensity projections, using conventional microscopy (a) and structured-illumination microscopy (b), through a 244-nm-thick subset of the data (two sections). (Insets) two parallel microtubules spaced by 125 nm (arrows) are well resolved in the structured illumination reconstruction, but unresolved by conventional microscopy. The image contrast of the inset in a has been adjusted, for easier comparison. (c) Cross-eyed stereo view of projections through the structured-illumination reconstruction. The data value of each voxel controlled both the brightness and the opacity of that voxel in the rendering. A video of this reconstruction is available on the journal web site (Movie S2).