Figure 7.

LRE analysis of eleven cDNA targets amplified with five reaction formulations. Summaries for LRE analysis of a total of 134 amplification profiles (each profile an average of four replicate amplification reactions) generated from eleven cDNA targets quantified using five reaction formulations (see Figure 2 and additional file 3 for additional details). (A) Average linear regression correlation coefficients (r²). (B) Average E_max. (C) Average ΔE. Note that ΔE is dependent on the optics of the assay and are thus not directly comparable across different formulations and gain settings. (D) Average F₀ derived from the average of F₀ values encompassed by the LRE window (see Figure 4 for details). QT: QuantiTect, DyNa: DyNAmo; FV: FullVelocity, SG: SYBR Green I, SD: standard deviation, n: the number of runs, na: not applicable (only one run conducted), nd: not determined.