Table 1.

LRE-based absolute quantification of eleven cDNA targets using five enzyme formulations.

Gain:	X8	X8	X2	X2	X1
OCF:	1092	1155	704	778	345
Enzyme:	QT 0XSG	QT 0.2XSG	DyNa 0.5XSG	DyNa 1.5XSG	FV 2.0XSG	LRE-No Average	CV (+/-)
Target
CHS K3K4	6,143	7,323	5,658	8,500	11,598	7,844	30.2%
SE K3K2	4,828	5,444	5,103	6,363	nd	5,435	12.3%
PHB K3K2	2,951	3,292	2,646	3,733	2,956	3,116	13.3%
EMF2 K2K3	2,301	3,256	3,094	2,752	nd	2,851	14.8%
NPR1 K1K4	1,872	1,973	1,565	1,657	2,334	1,880	16.0%
ANT K3K4	970	1,390	756	1,139	1,446	1,140	25.3%
FLF K3K4	661	553	466	596	nd	569	14.4%
HAP3C K3K4	145	198	254	255	nd	213	24.7%
ABI3 K3K4	66	105	129	122	48	94	37.8%
AP3 K3K4	38	52	61	34	22	41	37.3%
Lec2 K3K4	6	14	12	12	9	11	31.4%
						Av. CV:	23.4%

Following LRE analysis (summarized in Figure 7), the F_C readings encompassed by the LRE window were converted to F₀ (see Figure 3) and the average F₀ value converted to the number of target molecules (LRE-N₀) using the OCF generated by each respective reaction formulation (see Figure 6 and additional file 3 for more details). The gain setting was adjusted to prevent saturation of the photomultiplier (see Figure 2). Gain: the photomultiplier gain setting, OCF: optical calibration factor (FU/ng dsDNA), QT: QuantiTect, DyNa: DyNAmo; FV: FullVelocity, SG: SYBR Green I, SD: standard deviation, nd: not determined.