Table 2.
Absolute quantification based upon conversion of Ct to F0 via E max and Ft.
| Gain: | X8 | X8 | X2 | X2 | X1 | ||
| OCF: | 1092 | 1155 | 704 | 778 | 345 | ||
| Enzyme: | QT 0XSG | QT 0.2XSG | DyNa 0.5XSG | DyNa 1.5XSG | FV 2.0XSG | LRE-No Average | CV (+/-) |
| Target | |||||||
| CHS K3K4 | 5,876 | 7,562 | 5,564 | 9,188 | 10,813 | 7,801 | 28.5% |
| SE K2K3 | 4,874 | 5,919 | 5,255 | 7,485 | nd | 5,883 | 19.6% |
| PHB K3K2 | 2,653 | 3,405 | 2,730 | 3,916 | 2,888 | 3,119 | 17.1% |
| EMF2 K2K3 | 2,256 | 3,271 | 2,960 | 2,821 | nd | 2,827 | 15.0% |
| NPR1 K1K4 | 2,039 | 2,070 | 1,634 | 1,892 | 2,369 | 2,001 | 13.4% |
| ANT K3K4 | 923 | 1,288 | 972 | 1,342 | 1,450 | 1,195 | 19.6% |
| FLF K3K4 | 442 | 651 | 489 | 665 | nd | 562 | 20.1% |
| HAP3C K3K4 | 148 | 195 | 255 | 285 | nd | 221 | 27.7% |
| ABI3 K3K4 | 65 | 108 | 139 | 139 | 46 | 100 | 42.6% |
| AP3 K3K4 | 32 | 54 | 40 | 39 | 26 | 38 | 27.6% |
| Lec2 K3K4 | 3 | 14 | 11 | 11 | 8 | 10 | 41.7% |
| Av. CV: | 24.8% |
Ct values generated by the amplification profiles summarized in Figure 7 were converted to F0 using equation 6, and then converted to target molecule number via the OCF as used for LRE quantification (see text and additional data file 3 for additional details).