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Dependence of polymer appearance on the [PIN] status. Plasmids encoding the 70Q, 85Q, 131Q, 50QY, and 76QY proteins were introduced into the [PIN+], [pin-], and Δrnq1 cells, and transformants were grown for 30 or 100 (*) generations. Cell lysates were analyzed by SDD-AGE with blot immunostaining for Sup35M. Molecular mass standards are indicated in kilodaltons.
