FIGURE 3.

The toxicity of LqhαIT is enhanced by the ΔΔβ-toxins. Dose-response curves for toxicity of LqhαIT upon injection to blowfly larvae in the presence of increasing concentrations of ΔΔBj-xtrIT or ΔΔCss4 (in ng/100 mg of body weight). Forty larvae were used to generate each data point, which represents the ED₅₀ equivalent of LqhαIT in the presence of the indicated dose of ΔΔBj-xtrIT or ΔΔCss4. The half-maximal doses of ΔΔBj-xtrIT or ΔΔCss4 (EC₅₀) that increase the toxicity (decrease in ED₅₀) of LqhαIT are 5.2 and 9.6 ng/100 mg, respectively. Maximal enhancement in LqhαIT toxicity (∼600%) is achieved in the presence of less than 1 μg of ΔΔBj-xtrIT or ΔΔCss4.