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Ligand-induced optical changes of hinge-T QFR enzyme (25 °C). A and B, difference spectra show the effect of malonate (green;10 mm) fumarate (blue;10 mm), and OAA (magenta;2 mm) on the spectrum of fully oxidized FrdA T234A enzymes at pH 7.0 (A) and 9.0 (B). The concentrations of ligands were 10 mm malonate, 10 mm fumarate, and 2 mm OAA. C, OAA induced spectral changes of wild type QFR at pH 7.0 (black) and 9.0 (magenta). Protein concentration of wild type and hinge-T QFR was 4.9 μm.
