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Immunoflourescent staining of Bcl-2 and Bcl-2 antanogene-1 in breast tumor tissue. (a) Cytoplasmic Bcl-2, (b) cytoplasmic and nuclear Bcl-2 antanogene-1 (Bag-1) and (c) nuclear Bag-1 staining in a breast cancer histospot – using cytokeratin to the define tumor mask, using 4',6-diamidino-2-phenylindole to define the nuclear compartment, and using Cy5 for identifying the target (Bcl-2 and Bag-1).
