Table 3.

Summary of mutants by gene

Genea	Mutantb	Proteinc	DNAd	EMe	Function
J6R	Cts51, Cts53, Cts65, Dts85 Cts27, Cts29, Cts32, Cts47, Cts62, Dts10, Dts14,	defective late	positive	viroplasm - IV	RNAP (147 kDa); rpo147	Gene Expression
A24R	Dts28, Dts49, Dts52, Dts60, Dts66, Dts86, Dts90, Dts94	defective late	positive	viroplasm - IMV	RNAP (132 kDa); rpo132
A29L	Dts16, Dts17	not done	not done	not done	RNAP (35 kDa); rpo35
J4R	Cts7, Cts20, Dts44	defective late	positive	viroplasm	RNAP (22 kDa); rpo22
D7R	Cts21, Ets45	defective late	positive	viroplasm	RNAP (18 kDa); rpo18
G5.5R	Dts33	not done	not done	not done	RNAP (7 kDa); rpo7
H4L	Cts1, Cts31, Cts55, Cts58, Dts78	normal	positive	IMV	RAP94
D6R	Cts46, Dts95, Ets93	normal	positive	not done	VETF subunit
D11L	Cts36, Cts50, Dts61, Ets17	defective late	positive	not done	ATPase/NPH-I
D1R	Dts36, Dts50	defective early	negative	not done	VTF and capping enzyme subunit/ VITF
D12L	Dts96	normal	positive	viroplasm	VTF and capping enzyme subunit/ VITF
I8R	Cts10, Cts18, Cts38, Cts39, Cts44, Cts57, Dts67	normal	positive	IMV	RNA helicase/NPH-II
A1L	Cts63	defective late	positive	IVN	VLTF 2
A18R	Cts4, Cts22, Cts23	abortive late	positive	viroplasm	ATPase/DNA helicase/transcript release factor
G2R	Cts56, Dts22, Dts42	defective late	positive	viroplasm	positive transcription elongation factor
E9L	Cts42, Dts18, Dts20, Dts83, Dts97	early only	negative	negative	DNA polymerase	DNA Replication
A20R	Dts48	not done	negative	not done	DNA polymerase processivity factor
D5R	Cts17, Cts24, Dts12, Dts38, Dts56, Ets69	early only	negative	not done	ATPase/DNA primase
D4R	Dts27, Dts30	early only	negative	not done	uracil DNA glycosylase
B1R	Cts2, Cts3, Cts25, Dts15	early only	negative	not done	protein kinase
H5R	Dts57	early only	negative	negative	DNA replication/transcription/morphogenesis
A10L	Cts13, Cts64, Dts2	normal	positive	crescents	p4a	Structure/Assembly
A3L	Cts8, Cts26, Dts77	normal	positive	IVN	p4b
E6R	Cts52, Dts41, Dts80	normal	positive	IMV	virion core protein
E8R	Cts19, Dts23, Dts25	normal	positive	viroplasm - IMV	virion core protein
E11L	Cts49, Dts19	normal	positive	IMV	virion core protein
D2L	Ets52, Ets94	normal	positive	crescents	virion core protein
D3R	Cts5, Cts35	normal	positive	crescents	virion core protein
A30L	Dts45, Dts46	normal	not done	crescents	virion core protein
G7L	Cts11, Cts41, Dts68, Dts89	normal	positive	viroplasm	virion core protein
J1R	Cts45	normal	positive	crescents	virion membrane protein
A13L	Cts40	normal	positive	IV	membrane phosphoprotein
G3L	Cts60	normal	positive	EEV	entry/fusion complex
A28L	Cts6, Cts9	normal	positive	IMV	entry/fusion complex
F10L	Cts12, Cts15, Cts28, Cts54, Cts61, Dts11	normal	positive	viroplasm - IV	virion protein kinase
D13L	Cts33, Cts43, Dts9, Dts62, Dts88, Ets101	normal	positive	viroplasm	crescent scaffold protein
I7L	Cts16, Cts34, Dts4, Dts8, Dts35, Dts93	normal	positive	IVN	virion core proteinase
F13L	Cts30, Cts48	normal	positive	not done	EEV membrane protein
LM12, 32 dbl	Cts37	not done	not done	not done	not applicable
J3R-J4R dbl	Dts40	not done	not done	not done	not applicable
LM19, 21 dbl	Dts47	not done	not done	not done	not applicable
LM23, 26 dbl	Dts71	not done	not done	not done	not applicable
J6R, A36R dbl	Dts82	not done	not done	not done	not applicable

^adbl = double mutant, the smallest fragments producing rescue are indicated.

^bgenes mapped by marker rescue are underlined; all others are assigned to the indicated gene by complementation.

^cviral protein synthesis at the non-permissive temperature assayed by metabolic labeling and gel electrophoresis. Normal = indistinguishable from wild type; defective early = decreased synthesis of early proteins; early only = extended early protein synthesis, no late protein synthesis; defective late = decreased or delayed late protein synthesis; abortive late = late shut off of all protein synthesis. See (Condit and Motyczka, 1981; Condit et al., 1983).

^dviral DNA replication at the non-permissive temperature

^eelectron microscope phenotype at the non-permissive temperature. Entries denote the most mature normal structure visualized. Abnormal structures may be present as well. Negative = no evidence of viral infection; IV = immature virions; IVN = immature virions with nucleoids; MV = mature virions. Some groups display a range of phenotypes as indicated.