Figure 1.

Primary sequence alignment of the semaphorin genes with hanatoxin. (A) Diagram of type III semaphorin genes (the secreted semaphorins). The three major domains, semaphorin domain (Sema), Ig-like domain (Ig), and carboxyl-terminal domain (C), are indicated. The site with sequence homology to hanatoxin (HTLS) is indicated. (B) Primary sequence alignment of the products of the two most homologous semaphorin genes, Sema 3A and Sema 3D, with hanatoxin (amino acids 9–35). (C) Sequence alignment of those secreted semaphorin gene products with less homology to hanatoxin. (D) Primary sequence alignment of four membrane-bound semaphorin. * indicates identity, : indicates high similarity, and . indicates low similarity.