Regional distribution of type IIBrain and type III in adult human brain. Northern blots containing 2 μg poly-A+ RNA per lane from human brain were hybridized with a PCR-generated probe corresponding to the less conserved 3′ ends of type IIBrain and type III. (A) Lanes: 1, cerebellum; 2, cortex; 3, medulla; 4, spinal cord; 5, occipital lobe; 6, frontal lobe; 7, temporal lobe; 8, putamen. (B) Lanes: 1, amygdala; 2, caudate nucleus; 3, corpus callosum; 4, hippocampus; 5, whole brain; 6, substantia nigra; 7, subthalamic nucleus; 8, thalamus.