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Northern blot analysis. Northern blot analysis of CYP26 (RA hydroxylase), FGF-4, FGF-5, and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) mRNAs. CCE cells were cultured as in Fig. 1, and 50 nM retinol (A) or 1 μM retinol (B) was added 16 h before RNA harvest. The Northern analyses were performed three times with different RNA preparations with similar results.
