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Actinomycin D treatment of CCE. Cells were cultured as in Fig. 1, and 50 nM retinol, plus or minus 2 μg/ml actinomycin D, was added for 4 or 8 h before cell harvest and RNA preparation. RNA was hybridized with CYP26 and GAPDH. Lane 8 had a lower signal because the cells were beginning to be affected by actinomycin D toxicity at this 8-h time point (+Actinomycin D, −LIF). This experiment was performed twice with identical results.
