Figure 2.

The nucleus inside the capillary and the resulting equivalent circuit. The electrical resistance detected by the nuclear hourglass technique is composed of the resistance of the two NE segments, R_NE1 and R_NE2, and the resistance of the interior of the nucleus, R_Chromatin, in series. The parallel resistor, R_shunt, models the possibility of a substantial amount of the current I bypassing the nucleus through the narrow gap between the inner wall of the capillary and the nucleus. The surface area of the NE segments corresponding to R_NE1 and R_NE2 is calculated by using the geometrical parameters r (the radius of a hypothetical sphere segment) and h (its height) as indicated.