Figure 1.

(A) Deduced amino acid sequence of the CHK1 gene (CH_MAPK), compared with other fungal MAPK sequences (MG_PMK1, M. grisea Pmk1; SC_KSS1 and SC_FUS3, yeast Kss1 and Fus3, respectively). A consensus signature of the YERK1 subfamily is indicated (box) and is absent in MG_MPS1, Mps1 of M. grisea, which belongs to the YERK2 subfamily. (B) Phylogenetic tree based on amino acid sequence alignments of fungal MAPK homologs. The tree was constructed by using phylip (Phylogeny Inference Package), version 3.57c. The protdist subprogram (Categories distances option) was used to compute the distance matrix, and the tree and the indicated distances (multiplied by 100) were obtained by using the fitch (Fitch–Margoliash method) subprogram.