HT-transcript accumulation in SC N. alata cv Breakthrough. Developing anthers (lanes 1–4) and styles (i.e., stigma plus style, lanes 5–8) were dissected from buds of the indicated sizes. G + Y, styles at anthesis; G, isolated stigmas at anthesis; Y, isolated styles at anthesis; Pt, petals; Sp, sepals; St, stems; Lf, leaf. Total RNAs (10 μg/lane) were blotted and hybridized with 32P-labeled HT-cDNA.