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Sensitivity of detection of Blepharisma japonicum. Sensitivity of detection of Blepharisma japonicum by PCR amplification. Lanes denoted M contain molecular size markers – figures refer to sizes in base pairs. Dilutions of target DNA were amplified as follows: undiluted (lane 1), 1/5 (lane 2), 1/25 (lane 3), 1/125 (lane 4), 1/625 (lane 5), 1/3125 (lane 6). Lane 7 contains undiluted Paramecium caudatum DNA as a negative control.
