Figure 1.

B-induced changes in cytosolic, chloroplast, and nuclear Ca²⁺. All seedling batches were exposed to 10 s of B, and chemiluminescence was allowed to decay before [Ca²⁺]_c measurement commenced. (a) The averaged transient of [Ca²⁺]_c from 50 A. thaliana measurements and 50 N. plumbaginifolia measurements. Luminescence was measured every 0.2 s for over 3 min, and each point was converted to [Ca²⁺]_c before averaging. (b and c) N. plumbaginifolia expressing aeq in the chloroplast (n = 20; b) and in the nucleus (n = 20; c). (e and d) The effect of 200 μM thapsigargin (d) and 3 mM lanthanum chloride (e) on the cytosolic response in Arabidopsis. ⋄, light-treated; ×, dark control (the same plants before stimulation).