Figure 3.

Typical gelatin zymography analyses of gelatinase A (MMP-2) and gelatinase B (MMP-9) expression in artificial tear group rat and in the test groups were performed. Corneal conditioned medium samples from debrided (OD) and intact (OS) corneal epithelium of each group were analyzed. 1. Tears OD; 2. Tears OS; 3. Levofloxacin 0.5% OD; 4. Levofloxacin 0.5% OS; 5. Ciprofloxacin OD; 6. Ciprofloxacin OS; 7. Ofloxacin OD; 8. Ofloxacin OS. MMP-9 (92 kDa) and MMP-2 (72 kDa) expression were up regulated in fluoroquinolone treated corneas and were present at basal level in unwounded corneas receiving artificial tears. Corneal epithelium debridement up regulated the expression of MMP-9 and MMP-2 in all test groups.