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. Author manuscript; available in PMC: 2009 May 9.
Published in final edited form as: Mol Cell. 2008 May 9;30(3):325–335. doi: 10.1016/j.molcel.2008.02.028

Figure 4. Kinetics of 3’ non-homologous tail appearance and removal in SSA.

Figure 4

A. qPCR-based 3’-flap detection assay. Direct repeats (grey) and primers used for qPCR (arrows) are shown. B. Kinetics of 3’-flaps in tNS1379 (wild type) and various mutants. Genomic DNA from various mutants was digested by Sau3AI, and used for qPCR with primer sets that anneal to a 3’-flap. PCR results at multiple time intervals after HO expression using a primer set that anneal to a 3’-flap were normalized by control PCR with primers that anneal to ACT1 or PRE1, were shown to indicate kinetics of 3’-flap formation and removal. Data represent means ± s.d. from at least three independent experiments.