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. Author manuscript; available in PMC: 2009 May 9.
Published in final edited form as: Mol Cell. 2008 May 9;30(3):325–335. doi: 10.1016/j.molcel.2008.02.028

Figure 6. Interaction between Rad1 and Saw1 is critical for 3’-flap removal in recombination.

Figure 6

A. Positions of deletions in saw1 mutants. B. The effect of saw1 mutations on 3’-flap removal was tested using plasmid based flap removal assay with pFP120 carrying 308 and 610 bp of flaps. The percent plasmid retention were plotted for SAW1 deletion carrying wild type SAW1, saw1Δ18–24 or saw1Δ159–169 mutations. Data represent means ± s.d. from at least three independent experiments. C. Pull-down assay was performed to detect interaction between GST-Saw1 and GST-Saw1 mutants with Rad1 Msh2-TAP, Rad52-13Myc proteins. D. The effect of saw1 mutations on SSA between 205 bp ura3 direct repeat sequences flanked by a HO endonuclease-induced DSB. Southern blot hybridization of BglII digested genomic DNA with the radiolabeled probe next to the URA3 gene was performed to detect SSA product formation in SAW1 gene deletion strain expressing saw1 mutants from yeast centromeric plasmid.