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. Author manuscript; available in PMC: 2009 Jun 20.
Published in final edited form as: Virology. 2008 Apr 28;376(1):112–123. doi: 10.1016/j.virol.2008.03.022

Fig. 6.

Fig. 6

Sucrose gradient analysis of NHS-treated MuV particles. Purified MuV was incubated alone (panel A) or with NHS (panel B) for 60 min at 37 °C and then analyzed by centrifugation through 15–60% sucrose gradients. Fractions were collected and analyzed for virion proteins by western blotting with antiserum raised against the SV5 P protein which cross-reacts with MuV P protein (top and middle panels). A cross-reactive band of unknown origin is seen above the position of the P protein. C) Fractions 2–13 and 30–40 from the NHS-treated virion gradient were also analyzed by Western blotting for the presence of C3. The arrow indicates a high molecular weight protein that is not found in purified proteins in the marker lanes containing fragments of C3 and C3b.