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. Author manuscript; available in PMC: 2008 May 29.
Published in final edited form as: J Biol Chem. 2006 Oct 20;282(1):397–406. doi: 10.1074/jbc.M605333200

FIGURE 3. Fractionation of fluoresceinated bacteria.

FIGURE 3

OKN3 (fepA) harboring pITS23 (fepA+; lanes 1, 4, 7, and 10) or pFepAG54C (lanes 2, 5, 8, and 11) and OKN13 (fepA, tonB)/pITS23 (lanes 3, 6, 9, and 12) were grown in MOPS media to late log, pelleted by centrifugation and lysed in a French press. Their cytoplasm/periplasm (cyto/peri), IM and OM fractions were collected, resolved by SDS-PAGE, scanned for fluorescence (right panel), and the same gel was then stained with Coomassie Blue (left panel). The molecular weights of the outer membrane proteins Fiu (83 kDa), FepA (81 kDa), CirA (74 kDa), OmpF/C (36 kDa), and OmpA (denatured (33 kDa) and native 28 kDa)) are shown (respectively, top to bottom). Lane 13 shows a fluoresceinated lysate of OKN3/pFepAG54C, without any fractionation.