Figure 2.

(A) Detection of introduced genes. Genomic DNAs (10 μg) isolated from the control (lane C1 and C2) and the transgenic Oc cells harboring the antisense (lane A1 and A2), sense (lane S1 and S2), or mutated OsPSK cDNA (lane T1 and T2) were digested with BamHI. The generated fragments were hybridized with the labeled OsPSK cDNA. Bands derived from the endogenous OsPSK gene and the introduced OsPSK cDNA are indicated by black and striped arrowheads, respectively. (B–D) Comparison of growth of control and transgenic Oc cells. Control or transgenic Oc cells were transplanted into fresh MS medium containing 1.0 mg/liter 2,4-dichlorophenoxyacetic acid and 0.2% Gelrite. Photographs were taken 2 weeks after culturing at 25°C under light. (B) S2 sense transformants. (C) A2 antisense transformants. (D) C1 control Oc cells.