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DNA blot analyses of the OsPSK gene. (A) Genomic DNA (10 μg) isolated from rice Oc cells was digested with either BamHI (lane Ba), EcoRI (lane Ec), XbaI (lane Xb), or XhoI (lane Xh) and probed with the labeled full length cDNA at 65°C. (B) DNA blot analysis of the species distribution of OsPSK homologs. Genomic DNAs (10 μg) from Arabidopsis (1), asparagus (2), carrot (3), and zinnia (4) were digested with BamHI (lane Ba) or EcoRI (lane Ec) and hybridized with the same probe at 55°C.
