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. Author manuscript; available in PMC: 2008 May 29.
Published in final edited form as: Endocrinology. 2004 Oct 21;146(2):816–824. doi: 10.1210/en.2004-1153

Fig. 8.

Fig. 8

Sidedness of the acidification. Experiments were done as in Fig. 4. hECE cells were grown in steroid-free medium (S.F.M.) plus 10 nM 17β-estradiol. Thirty minutes before assays bafilomycin A1 (1 μM) was added to either the CL or L solution. Determinations of pHo in the CL-pHo and L-pHo were described in Materials and Methods. Shown are means (± SD) of three to five repeats per point 30 min after mounting filters for assays. a, b, c, P < 0.01, compared with CL-pHo; d, P < 0.01, compared with c.