Fig. 3.

A: The effect of antisense PML on survival of serum starved NIH 3T3 cells. Oligonucleotides were added to a total concentration of 3 μM. Cells were grown in 12 well plates in 10% FBS/DMEM, pre-treated for 1 h with the appropriate oligonucleotide or with media only. Cells were then rinsed with serum free media and new serum free media was added. After 18 h, cells were harvested, and counted. Experiments were carried out in quadruplicate and four fields were counted for each. Error bars indicate standard deviations. B: Protein expression levels of PML were monitored using standard techniques in cells as treated in A. Total cell lysates were used where the total protein concentration was normalised prior to loading onto gels. Standard Western blots were obtained and probed with a PML polyclonal antibody (see Section 2). –Se indicates serum withdrawn, +Se, with serum, S, serum withdrawn cells treated with sense oligonucleotide and AS with antisense.