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Effect of age on senescence-associated β-galactosidase (SA-β-gal)-positive human marrow stromal cells (hMSCs). hMSCs isolated from 17 subjects were stained for SA-β-gal. Value of percent positive cells is expressed for each subject as mean ± SD with 4 replicates. Inset: Frequency of hMSCs positive for SA-β-galactosidase was 4-fold higher in the group of subjects older than 55 years (n=12) than in the group younger than 50 years (n=5) (p<0.001, Mann-Whitney test).
