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. 2008 Jun 11;3(6):e2376. doi: 10.1371/journal.pone.0002376

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Muller et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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LP DC were prepared, intracellular cytokine staining was performed and the cytokine expression was analyzed by FACS (A). For analysis of cytokine mRNA expression, LP DC were enriched by FACS sorting and expression of IL-6 and TNF-α mRNA was analyzed by quantitative RT-PCR (B). * p<0.05 compared to B. vulgatus mpk mono-colonized mice.