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. 2008 Jun 11;3(6):e2401. doi: 10.1371/journal.pone.0002401

Figure 4. Foxc proteins interact with VEGF signaling in the activation of the Dll4 and Hey2 promoters.

Figure 4

(A) The VEGF-induced PI3K pathway activates the Dll4 and Hey2 promoters. BAECs transfected with DLL4-LUC or Hey2-LUC at 24 h after transfection and serum starvation were treated with either PI3K inhibitors (Wortmannin and LY294002) or MEK inhibitors (PD98059 and U0126) along with VEGF (50 ng/ml) for additional 24 h. Luciferase activity was assayed at 48 h after transfection. (B) Transcriptional activity of Foxc proteins is modulated by VEGF-mediated PI3K and MEK pathways. BAECs transfected with Foxc expression vectors along with DLL4-LUC or Hey2-LUC at 24 h after transfection and serum starvation were treated with VEGF (50 ng/ml) in the presence of Wortmannin or PD98059 for additional 24 h. Luciferase activity was assayed at 48 h after transfection. Values are means+s.d. of 3 experiments in triplicates. Statistical significance was determined by Student's t-tests. (*p<0.05, **p<0.01 versus control cells treated with VEGF)