Figure 5.

Effect of ATP on ERK-1/-2 (p44/p42) and Elk-1 in the absence or presence of PD98059 and staurosporin. To examine the role of ATP on MAPKs in IOSE-29, the cells were pretreated with 50 μM PD98059 or 0.1 μM staurosporin for 30 min, followed by treatment with 100 μM ATP for 10 min. The P-MAPK normalized by T-MAPK was analyzed by immunoblot analysis, and Elk-1, a downstream pathway of ERK-1/-2, was measured by in vitro MAPK assay, respectively. [Reproduced, permission with, from: Choi K-C, Tai C-J, Tzeng C-R, Auersperg N, Leung PC; "Adenosine triphosphate (ATP) activates mitogen-activated protein kinases (MAPKs) in neoplastic ovarian surface epithelium (OSE) cells" in: Biol Reprod 2003, 68: 309–315. Copyright from Society for the Study of Reproduction]