Figure 2.

Complementation of the camalexin-deficient phenotype (A), enhanced bacterial growth phenotype (B), and the PR-1 transcript accumulation phenotype (C) of pad4–1 by cosmids 8 and 21. Wild-type (Col), pad4–1, and transgenic pad4–1 containing cosmid 8 or cosmid 21 were infected with Psm ES4326. Camalexin levels in infected leaves were determined 48 hr after infection. Bacterial titer was determined 3 days after infection, and PR-1 mRNA levels were determined 36 hr after infection. For A and B, each bar represents the mean and SD of six replicate samples. In C, the 18S rRNA probe was used to evaluate uniform loading. Similar results were obtained in another independent experiment.