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After infection by Psm ES4326, PAD4 transcript levels are very high in wild-type plants and greatly reduced all pad4 mutant alleles. Leaves from wild-type (Col) and all four pad4 mutants were excised 0, 6, 12, 24, 36, or 48 hr after infection. Mg indicates leaves mock-inoculated with 10 mM MgSO4 and harvested after 36 hr. Similar results were obtained in another independent experiment.
