Figure 3.
IFN-γ treatment affects the quality but not quantity of the allogeneic T cell MLR response stimulated by BCG matured DC DC were treated with 4.1 × 105 CFU/ml of BCG alone, BCG plus 1000 U/ml IFN-γ or left untreated (immature) for 24 h prior to mixed leukocyte culture. Varying numbers of DC were added to 2 × 105 enriched T cells in triplicate wells of a 96-well microculture plate and cultured for 5 days. The cells were pulsed with 3H-thymidine for an additional 24 h before harvesting and quantitation of incorporated radioactivity in a scintillation counter (A). Immediately preceding the 3H-thymidine pulse, culture supernatants were collected for evaluating IFN-γ secretion (B). Results shown in each panel are from a representative experiment out of three similar experiments.