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Apoptotic levels in SPD-treated cells. SPD treatment (10-6 M) significantly increased the level of apoptosis in MCF-7 cells when compared to untreated controls, as judged by apoptotic morphology by nuclear staining and DNA fragmentation by TUNEL assay described in the Experimental Procedures. Increased levels were observed till 72 hours of SPD treatment. Results were presented as the means ± SD of 6 independent experiments.
