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The C-terminal α-helix of histone H4 is dispensable for nucleosome assembly. As in Fig. 2B, Lanes 1–4 and 9–12, protein levels in the unfractionated embryo; lanes 5–8 and 13–16, protein levels in chromatin. Lanes 1, 5, 9, and 13, no RNA injected; lanes 2, 6, 10, and 14, wild-type H4 RNA; lanes 3 and 7, C-terminal deletion mutant CΔ80-102; lanes 4 and 8, N-terminal deletion mutant NΔ1-45; lanes 11 and 15, CΔ75-102; lanes 12 and 16, CΔ70-102.
