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. 1996 Nov 12;93(23):12780–12785. doi: 10.1073/pnas.93.23.12780

Figure 4.

Figure 4

C-terminal DNA contacts and the long central α-helix of H4 are important for nucleosome assembly. Lanes 1–5, protein levels in the unfractionated embryo; lanes 6–10, protein levels in chromatin. Lanes 1 and 6, no RNA; lanes 2 and 7, H4; lanes 3 and 8, residues 60–63 mutated to glycine; lanes 4 and 9, epitope-tagged amino acids 32–80; lanes 5 and 10, arginines 35 and 36 mutated to alanine. The bar indicates the position of tagged H4 protein.