C-terminal DNA contacts and the long central
α-helix of H4 are important for nucleosome assembly. Lanes 1–5,
protein levels in the unfractionated embryo; lanes 6–10, protein
levels in chromatin. Lanes 1 and 6, no RNA; lanes 2 and 7, H4; lanes 3
and 8, residues 60–63 mutated to glycine; lanes 4 and 9,
epitope-tagged amino acids 32–80; lanes 5 and 10, arginines 35 and 36
mutated to alanine. The bar indicates the position of tagged H4
protein.