Figure 5.

Domains of histone H3 essential and dispensable for nucleosome assembly. (A) Newly synthesized proteins in the embryo are indicated (lanes 1–8, 11–13), as in their incorporation into chromatin (lanes 9, 10, and 14–16). Detection is by radiolabeling (lanes 1–10) or by immunoblotting (lanes 11–16). The arrowhead indicates the N63 deletion. Note that endogenous histone synthesis is visualized in lanes 1, 8, and 10 in the absence of any expression of mutant H3 since no mRNA is injected (No RNA). (B) Indicated proteins synthesized in the embryo (lanes 1–6) are shown incorporated into chromatin (lanes 7–12). Detection is by immunoblotting.