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FCS detection unit. The plastic sheet was fixed on a heating block at 42°C. Ten samples were subjected to FCS laser beam one after another, which generated the volume element within these vessel (Inset) at a distance of 200 μm from the objective. The heating block was moved by computer, thus ensuring reproducible measurements. Fluorescence intensities of probe gag1 within the volume element were processed online by PC correlator card leading to autocorrealation curves.
