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Characterization of RNA amplification products by ELGA. Lanes 1 and 2: 3 μl of NASBA negative controls after 70 min (free horseradish peroxidase oligonucleotide probe). Lanes 3 and 4: 3 μl of NASBA reaction after 70-min incubation (2000 initial HIV-1 RNA molecules); two RNA products (108 and 145 bases, see Fig. 1) could be observed.
