Table 3.
Transcription factors or cofactors that interact with SF-1. For each interacting factor, the resulting functional interaction, the experimental approach used to evidence the physical interaction and the model promoter are indicated.
| Factor | Functional interaction | Physical interaction | Promoter | Bibliography |
| TreP132 | allows CBP/p300 recruitment | Co-IP, pull-down, two-hybrid | P450scc | [160,161] |
| GATA-4 | Independent of GATA binding to DNA | Pull-down | MIS | [106,150] |
| SOX9 | Increases transcription in the presence of SF-1 | Two-hybrid, pull-down, co-IP, EMSA | MIS | [105] |
| WT1-KTS | Activation by WT-1 is dependent on SF-1 binding to its site. No WT-1 binding to DNA | Two-hybrid, GST pull-down | MIS | [107] |
| Sp1 | Cooperation dependent on the cell type | Co-IP, two-hybrid, EMSA | StAR | [202] |
| Sp1 | Cooperation required for basal and cAMP-induced activity. Allows recruitment of CBP | Two-hybrid, EMSA | bovine P450scc | [153,162] |
| Egr-1 | Synergistic activation. Egr-1 expression is induced by GnRH. | Pull-down | LH-β | [155-157] |
| Ptx-1 | Synergy depends on SF-1 binding to DNA | Pull-down, two-hybrid | LH-β | [158,159] |
| CREB | Synergy in basal and cAMP-induced conditions. Allows CBP recuitment | Pull-down | α-Inhibin | [163] |
| CREB | Synergy in response to cAMP. FSH induces CREB and SF-1 phosphorylation | Not demonstrated | Aromatase | [86,164] |
| AP-1/c-jun | Synergy for P450scc and an artificial promoter activation | Pull-down | P450scc and SFRE-tk-CAT | [14,21] |
| TF-IIB | No determined | Pull-down | [14] | |
| C/EBPβ | C/EBP binding sites are required for activation by SF-1 | Pull-down | mStAR | [112] |
| USF | USF is required for SF-1 to activate rFSH-R promoter. Activation is inhibited by PKA. | Not demonstrated | rFSH-R | [209] |
| AR | AR represses LHβ transcription by interacting with SF-1 | Pull-down | bLHβ | [154] |
| DP103 | Inhibits SF-1 transcriptional activity by interacting with a proximal repression domain. | Co-IP, pull-down | P450scc, P450c21, progesterone production | [148,149] |
| P54nrb et PSF | Repression of basal and induced hCYP17 promoter activity | EMSA, co-IP | hCYP17 | [222] |
| WT1 | Aromatase transcription inhibition. Integrity of the SFRE is required | Not demonstrated | Aromatase | [223] |
| DAX-1 | Inhibits SF-1 transcriptional activity NCoR/Alien recruitment | Pull-down, two-hybrid | Synthetic promoters and SF-1 target genes | [107,151,152] |