Table 2.

Selection for base replacements of the finger 1 binding site of Zif268

DNA triplet	Sort	Amino acid at helix positions				Predicted net charge at pH 7.0
		−1	2	3	6
ANN	5	Q	G	S	G	0
		A	V	G	R	0
		E	S	E	R	−1
		G	G	Q	G	0
		E	L	N	S	−1
		V	Q	E	N	−1
		E	V	G	E	−2
		K	G	E	G	0
CNN	5	P	A	G	K	+1
		V	W	R	Q	+1
		G	E	K	G	0
		D	K	G	Q	0
		G	G	R	E	0
		S	H	R	A	+1
		G	D	E	R	−1
		L	D	R	D	−1
		E	R	G	G	0
GNN	5	T	Q	S	R	+1
		S	S	E	R	0
		E	Q	R	R	+1
		E	S	S	R	0
TNN	5	T	R	G	Q	+1
		T	G	G	K	+1
		S	A	V	T	0
		N	S	G	Q	0
		R	S	D	K	0
NAN	3	S	G	V	R	+1
		T	Q	G	R	+1
		K	A	T	R	+2
		R	A	A	R	+2
	5	S	G	G	R (3)	+1
		E	R	S	R	+1
	6	S	A	N	R	+1
		E	A	N	R	0
		S	G	G	R (4)	+1
		S	T	G	R	+1
		S	S	G	R	+1
		S	A	G	R	+1
		E	G	S	R	0
		E	G	A	R	0
NCN	3	D	W	G	R	+1
		S	T	G	R	+1
		D	W	S	R	0
		G	W	E	R	0
	6	R	S	E	R (4)	+1
		Q	G	E	R	0
		R	V	D	R	+1
		A	R	D	R	+1
		E	G	A	R	0
		E	G	S	R	0
		E	A	G	R	0
		A	A	Q	R	+1
NGN	3	S	G	G	R (2)	+1
		T	T	G	R	+1
		E	K	S	R	+1
	6	R	E	H	R	+1
		K	E	H	R	+1
		S	G	G	R (2)	+1
		T	S	G	R	+1
		T	G	G	R	+1
		T	F	G	R	+1
		E	S	K	R (3)	+1
NTN	3	E	T	S	R	0
		E	R	S	R	+1
		T	S	A	R	+1
		E	G	S	R	0
		E	T	S	R	0
		T	G	S	R	+1
	6	T	N	A	R	+1
		T	W	M	R	+1
		E	G	A	R (3)	0
		T	G	M	R	+1
		S	S	S	R	+1
		T	H	A	R	+1
		S	G	G	R (3)	+1