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. 1996 Nov 12;93(23):12845–12850. doi: 10.1073/pnas.93.23.12845

Figure 2.

Figure 2

Identification of a glycine-rich mRPD3-interacting domain in YY1. (A) Schematic drawing of YY1 and GST–YY1 fusion constructs. The glycine-rich region (mRPD3 binding domain) is shaded in gray. The location of activation, repression, and DNA binding domains on YY1 is from refs. 1, 7, and 26–28. The ability of each GST–YY1 fusion protein to bind mRPD3 is indicated (+ or −). (B) Representative autoradiogram of in vitro translated mRPD3 protein captured by GST–YY1 fusion proteins. Three experiments yielded consistent results. The input lane (lane 16) was loaded with one-third the amount of mRPD3 used in the binding reactions. The sizes of molecular weight markers are indicated to the left.